BIOL340 Cellular Membranes & Cytoskeleton

BIOL 340 Cellular Membranes & Cytoskeleton Questions

Biol 340 Cellular Membranes & Cytoskeleton Readings: EBC4e

Chapter 11 & 17 Technique: FRAP Figures: Ch. 11 -29 Ch.17 -2,-4, -5, -6, -8, -10, -11, -12, -14, -15, -16, -18, -19, -22, -26, -28, -29, -30, -31, -34, -38, -39, -40, -41, -42, -43, -44, 46

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− Describe how and where new phospholipids are made.

 − Explain the function of scramblases and flippases. − Looking at Fig 11-18:

? Name the major phospholipids that comprise cellular membranes

? Which phospholipids are typically on the inner leaflet; on the outer leaflet?

− Describe the major functions of membrane proteins − Describe the difference between an integral and peripheral protein

− Describe the structure of a transmembrane protein and how it associates with the membrane

− Describe the properties that allow a molecule to pass through the membrane vs. needing a transporter

− Explain the FRAP technique and what it measures

− Explain how lateral mobility of plasma membrane proteins can be restricted

− Explain how lateral mobility of plasma membrane proteins contributes to cell polarization

− Describe the functional significance of glycolipids and glycoproteins

− Look at questions 11-12a and 11-15 at the end of the chapter for their significance. membrane synthesis

 ? Review the information you collected for the organelle review sheet. Compare and contrast the properties of microfilaments (actin) to microtubules (MT) to intermediate filaments (IF) and relate them to their functions in the cell.

? Name an IF in epithelial cells, muscle and connective tissue cells, nerve cells and then one that is common to all animal cells. Again, describe at least one function of each IF in each of these cells.

 ? Describe the polymerization process of MT and what stabilizes them.

? Describe the concept of dynamic instability.

 ? Describe the importance of γ-tubulin. What would happen if it were not present in centrosomes?

? Describe how microtubules found in cilia and flagella differ from regular cellular MTs. Describe in general how the motors cause movement of these structures.

? Describe the structure and function of a cell cortex.

? Describe how actin polymerizes and undergoes treadmilling.

 ? List examples of actin binding proteins and their general function in how actin polymerization can cause forward movement of cells and in changing the cytoplasm from a gel state to a sol state.

 ? Describe the structure and function of molecular motors.

 ? Name two motors for MT and two for actin and their general functions.