mcb2289L Module 04 LearnSmart Lab Quiz

• Question 1

1 out of 1 points

In a negative stain, which of the following is stained?

: Cell wall

Cytoplasm

Background

Cell membrane

• Question 2

1 out of 1 points

In a differential staining technique, a ______ is used to remove the primary stain from the bacterial cell wall

: Mordant

Decolorizer

Secondary stain

Counterstain

• Question 3

1 out of 1 points

Why might it be important to perform a Gram stain on bacterial cells?

As part of the information used to choose an appropriate antibiotic treatment

To investigate the cell's organelles

In order to determine the species of an infectious agent

To make the bacterial grow more efficiently on nutrient media

• Question 4

1 out of 1 points

In the Gram stain, the chemical that fixes dye in or on cells by forming an insoluble compound in referred to as the _______________?

mordant

decolorizer

counterstain

primary stain

• Question 5

1 out of 1 points

In order to withstand hostile conditions and facilitate survival, some bacteria produce resistant structures known as:

: Ribosomes

Endospores

Centrioles

Flagella

• Question 6

1 out of 1 points

Staining allows for visualization of bacterial cells because it increases contrast during microscopic imaging?

: True

False

• Question 7

1 out of 1 points

The purpose of heat fixation is to _______.

: Adjust the morphology of the organisms on the slide

Encourage growth of bacterial cells on the slide

Inactivate and adhere microorganism placed on the slide

Harden the bacteria so that they are not destroyed by the staining procedure

• Question 8

1 out of 1 points

Gram negative cell walls contain a thin layer of peptidoglycan whereas Gram positive cell walls contain a thick layer of peptidoglycan.

: True

False

• Question 9

1 out of 1 points

During a simple stain, what will happen in the heat fixation step is omitted?

: The cells will not take up the dye

The cells will be destroyed by the staining process

The cells may wash off the slide

The cell morphology will change

• Question 10

1 out of 1 points

All of the following are true regarding bacterial smears EXCEPT

: Preparation of bacterial smears is required for bacterial staining procedures

Bacterial smears provide scientist with the means to look at microorganisms

Bacterial smears are used to treat infectious diseases

Preparation of smears disperses bacterial cells on a slide so that individual cells can be visualized under the microscope

• Question 11

1 out of 1 points

A simple stain provides enough information about a microbe to clearly identify its species:

: True

False

• Question 12

1 out of 1 points

________ is used to stain the endospore in the endospore staining procedure

: Methylene blue

Crystal violet

Malachite green

Safranin

• Question 13

1 out of 1 points

Staining techniques do NOT provide information on which of the following structures?

: Inclusions

Ribosomes

Capsule

Cell wall

• Question 14

1 out of 1 points

Encapsulated organisms are difficult to directly stain because ___

: The capsule easily reacts with most stains

The capsule prevents bacteria from adhering to the slide

The capsule is easily removed from the cell

The capsule is not permeable to most stains

• Question 15

1 out of 1 points

Spherical cells arranged in chains are called __________.

: Staphylococci

Streptobacilli

Spirillium

Streptococci

• Question 16

1 out of 1 points

The first dye applied in a differential staining technique is referred to as the counterstain

: True

False

• Question 17

1 out of 1 points

Gram negative cells appear purple after Gram staining whereas gram positive cells appear pink

: True

False

• Question 18

1 out of 1 points

In the Acid-fast stain, non-acid-fast bacteria appear pink under the microscope

: True

False

• Question 19

1 out of 1 points

Stains are commonly used for all of the following EXCEPT

: The pathogen identification process

The diagnosis of disease

The classification of organisms

The treatment of disease

• Question 20

1 out of 1 points

Which of the following diseases are caused by acid-fast bacterium:

: HIV

Influenza

Cholera

Tuberculosis (TB)