MCB2340C Module 01 Quiz Latest 2023

MCB2340C Section 04 General Microbiology  

Module 01 Quiz

Question 1

Rod-shaped bacteria, regardless of their ability to form endospores, are referred to as ___________.

1.Cocci

2.Bacilli

3.Spirillum

4.Vibrioid

Question 2

Chemically, proteins can function as

1.Energy storage

2.Information storage

3.Enzymes

4.Pigments

Question 3

__________ includes the organisms yeast and mold.

1.Algae

2.Bacteria

3.Fungi

4.Viruses

Question 4

After you dry your hands with a paper towel, where should you dispose of the paper towel?

1.Garbage can

2.Biohazard container

3.Sharps container

4.Empty sink

Question 5

The aseptic technique involves:

1.Wearing the appropriate PPE to protect yourself, the organism, and the environment from contamination.

2.Using sterilized media and equipment to prevent contamination of the organism.

3.Making sure air currents are not present that could bring contaminants in or out of your vessels.

4.All answers are true.

Question 6

The scientific method involves:

1.Planning an experiment to test your hypothesis.

2.Analyzing the results and concluding whether your hypothesis was correct.

3.Making sure your biases do not overrule the data collected.

4.All answers are true.             

Question 7

Which of the following industries use(s) microorganisms for critical steps?

1.Wine and beer

2.Cheese and yogurt

3.Wastewater

4.All answers are true

Question 8

Which of the following have either DNA or RNA but not both?

1.Archaea

2.Bacteria

3.Prions

4.Viruses

Question 9

Viruses are easily viewed with the brightfield microscope using the 100X objective lens and immersion oil.

1. True

2.False

Question 10

Thin, flexible spiral bacteria are called:

1.Cocci

2.Spirillum

3.Vibrio

4.Spirochete

Question 11

Fungi and some protozoa produce a compound called _____ which strengthens the cell membranes

1.cholesterol

2.ergosterol

3.hopanoids

4.estrogen

Question 12

The sharps container should be used to dispose of which of the following items?

1.Used paper towels

2.Used gloves

3.Bacterial culture plates

4.Used microscope slides

Question 13

Some bacteria strengthen the cell membrane by incorporating various ___________ compounds in it.

1.Cholesterol

2.Ergosterol

3.Hopanoid

4.Estrogen

Question 14

The aseptic technique involves:

1.Protecting yourself and others from contamination

2.Protecting your pure culture from contamination

3.Protecting your environment from contamination

4.All answers are true

Question 15

Cocci arranged in squares of four in irregular clusters are called:

1.Diplococci

2.Streptococci

3.Tetrads

4.Sarcinae

Question 16

Animal, plant, and algal cells produce a compound called __________ that strengthens the cell membranes.

1.Cholesterol

2.Ergosterol

3.Hopanoid

4.Estrogen

Question 17

Multicellular, parasitic organisms that spend most of their time in a mammalian host are called ________.

1.Protozoans

2.Fungi

3.Helminths

4.Algae

Question 18

The term _______________ describes the cloudy growth one sees in a broth after inoculation with a culture and overnight incubation.

1.Precipitate

2.Pellicle

3.Sediment

4.Turbidity

Question 19

Chemically, lipids can function as

1.Transport molecules

2.Hormones

3.Receptors

4.Structural components

Question 20

Biosafety level 3 involves

1.Microbes that are dangerous and exotic and often cause fatal disease

2.Organisms such as Mycobacterium tuberculosis

3.Protective suits with their own air supply and positive pressure relative to the outside

4.All answers are true

Question 21

Which type of media provides the best option for obtaining a culture with isolated bacterial colonies?

1.Sim tubes

2.Nutrient agar plates

3.Nutrient broth tubes

4.Semi-solid agar tubes

Question 22

The person considered to be the "father of microbiology" and who was the first to observe live bacteria and protozoans was:

1.Antony van Leeuwenhoek

2.Robert Hooke

3.Louis Pasteur

4.Joseph Lister

Question 23

Eukaryotic microorganisms include fungi, protozoans and algae.

1. True

2. False

Question 24

When first using a brightfield microscope to view a specimen, which objective lens should be the first you use?

1.100X

2.40X

3.10X

4.4X (scanning objective)

Question 25

The Centers for Disease Control guidelines suggest that you should scrub with soap for a minimum of _________ seconds

1.10

2.15

3.5

4.20

Question 26

The aseptic transfer from one glass

tube into another should be accomplished by doing which of the following actions to the tubes?

1.Hold the mouth of one tube in the flame while the inoculating tool is inserted for removal of organisms.

2.Remove the cap of one tube and place it on the table away from the flame.

3.Hold the bottom of the tube in the flame for 10 seconds before transferring the culture.

4.The mouth is passed through the flame right when opened and just before closing.

Question 27

You notice that your lab partner has the following items on the lab bench. Which would you suggest they remove before beginning the lab activity?

1.Notebook

2.Pencil

3.Starbuck's coffee

4.Safety goggles

Question 28

Starch is an example of a __________

1.Protein

2.Carbohydrate

3.Lipid

4.Nucleic acid

Question 29

When using a microscope, the ability to distinguish fine detail or discriminate between two very closely spaced objects is called:

1.Microscopy.

2.Resolution.

3.Clarification.

4.Magnification.

Question 30

Which item is used to support the growth of a microbial population?

1.Inoculating loops

2.Inoculating needle

3.Sterile water

4.Culture medium

Question 31

Into what type of container should used microbiology gloves be disposed of?

1.Garbage can

2.Biohazard container

3.Sharps container

4.Empty sink

Question 32

The scientific method doesn't involve:

1.Asking a question.

2.Forming a hypothesis.

3.Planning an experiment.

4.Making the data fit your hypothesis no matter what.

Question 33

Beadlike chains of cocci formed after cell division along a single axis are called:

1.Diplococci

2.Streptococci

3.Tetrads

4.Staphylococci

Question 34

If you look at an agar plate and you see a fuzzy colony apart from your original bacterial streak that has colonies that appear round and glossy, this is called contamination.

1. True

2. False

Question 35

Chitin is an example of a __________

1.Protein

2.Lipid

3.Carbohydrate

4.Nucleic acid

 

Module 01 Lab Quiz

Question 1

All these terms categorize microorganisms based on their temperature preference EXCEPT

Obligate halophile

Psychrophile

Mesophile

Psychrotroph

Question 2

If the heat-fixation step is omitted in a simple stain, what will happen?

The dye will not be taken up by the cells

The staining process will destroy the cells

The cells can wash off the slide

The cell morphology will change

Question 3

Gram-negative cell walls contain a thin layer of peptidoglycan, whereas Gram-positive cell walls contain a thick layer of peptidoglycan.

True

False

Question 4

All the following organisms are expected to grow within an anaerobe jar EXCEPT

Obligate anaerobe

Aerotolerant anaerobe

Obligate aerobe

Facultative anaerobe

Question 5

If a bacterium were placed in a hypertonic solution prepared by the addition of sucrose, which of the following would happen to the bacterium?

The bacterial cell would swell but not lyse because of the presence of the cell wall

The bacterial cell would not change

The bacterial cell would undergo osmotic lysis

The bacterial cell would undergo plasmolysis

Question 6

All the following are environmental factors that affect microbial growth EXCEPT

Temperature

Osmotic pressure

pH

Cell wall type

Question 7

A ___________ is used to remove the primary stain from the bacterial cell wall in a differential staining technique.

Mordant

Decolorizer

Secondary stain

Counterstain

Question 8

In the acid-fast stain, non-acid-fast bacteria appear blue under the microscope.

True

False

Question 9

An acidophile is an organism that has an optimal pH for growth below 5.5.

True

False

Question 10

All microorganisms use oxygen in their metabolism.

True

False

Question 11

The environmental conditions on human skin have NaCl levels slightly above that of most bacterial cells. What category of organism would most likely be part of the normal skin microflora?

A halotolerant organism

An obligate halophile

An obligate anaerobe

A psychrophile

Question 12

Capsule staining techniques stain the background and the vegetative cell of an incapsulated bacterium because the capsule doesn’t react with most stains.

True

False

Question 13

All these terms categorize microorganisms based on their temperature preference EXCEPT:

Mesophile

Psychrophile

Psychrotroph

Chemoautotroph

Question 14

All the following are found in most prokaryotic cells EXCEPT:

cell membrane

cytoplasm

cell wall

Nucleus

Question 15

A ____________ is used in the Gram stain procedure to complex with the dye and trap it within the cell of a bacterium with a thick peptidoglycan cell wall?

Mordant

Decolorizer

Counterstain

Primary stain

Question 16

Gram positive cells appear purple after Gram staining whereas Gram negative cells appear pink.

True

False

Question 17

___________________ is used to stain the endospore in the endospore staining procedure.

Methylene blue

Crystal violet

Malachite green

Safranin

Question 18

Heat-fixation is performed in slide preparation in order to _________________________________.

Fix the morphology of the organisms on the slide

Encourage bacterial growth on the slide

Inactivate and adhere microorganism placed on the slide

Protect the bacteria by hardening them to prevent destruction by the staining procedure

Question 19

Heating or steaming a fixed smear with the primary stain, malachite green, enhances the penetration of the stain into this structure.

Capsule

Cell wall

Chloroplast

Endospore

Question 20

A hypotonic solution is one with a solute concentration greater than the concentration which exists within the cell.

True

False

Question 21

If a bacterium were placed in a hypotonic solution prepared by the addition of sucrose, which of the following would happen to the bacterium?

The bacterial cell would swell but not lyse because of the presence of a cell wall

The bacterial cell would not change

The bacterial cells will undergo osmotic lysis

The bacterial cells will undergo plasmolysis

Question 22

The simple stain technique provides enough information about a microbe to identify its species reliably:

True

False

Question 23

As long as the normal enzymatic function is not compromised, a microorganism will grow across the range of pH.

True

False

Question 24

Organisms that require a lower level of molecular oxygen for their metabolism than what is typically found in the atmosphere, are called __________________?

Microaerophiles

Obligate aerobes

Facultative anaerobes

Aerotolerant anaerobes

Question 25

Where would you expect an obligate aerobe to grow in a fluid thioglycollate media tube?

The upper part of the tube

The lower part of the tube

Throughout the tube

The middle part of the tube